ANTHEPROT - Signal prediction
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The solvent accessiblity method (von Heijne, 1998) is useful to predict the location of :

The parameters of the methods are taken from the database of signal peptides. The occurence of amino acids at each position of a signal peptide was calculated for eucaryotic and procaryotic sequences separately. Then a frequency table was established and used to calculate the score o all windows (15 aa long) moving along the actual sequence. The most probable cleavage site was the highest score obtained.


How to make it with ANTHEPROT?
Since, you need only 1 sequence, the signal prediction can be obtained when only one sequence is loaded.